Invasive Fungal Disease highlighted at the 22 nd European Congress

Dr Klaassen reported results of a recent study in which a novel mixed-format real-time polymerase chain reaction (PCR) assay was used to identify wild type cyp51A and point mutations conferring azole resistance in A. fumigatus isolates. Hybridisation of a single strand DNA detection probe with single strand target gene DNA enabled the researchers to differentiate between susceptible and resistant strains, using fluorescent dyes. Four triazoleresistant isolates were identified, all of which contained an identical combination of mutations leading to multi-triazole resistance, and molecular testing results were totally concordant with phenotypic susceptibility testing.